骨科植入物局部生命化

骨科植入物局部生命化上海交通大學機械與動力工程學院生物醫學制造與生命質量工程研究所骨科植入物的類型脊柱類關節類創傷類CombustorLinerRobotArmAcetabularCupBlastResistantPanel金屬3D打印技術標準系列產品多孔鈦合金個體化植入物個體化植入物金屬3D打印技術開辟了骨科植入技術的新紀元以激光為能量源以電子束為能量源金屬3D打印技術金屬3D打印技術德國ConceptLaserM2激光熔融成型技術（SelectiveLaserMelting,SLM）瑞典ArcamA1電子束熔化成型技術（ElectronBeamMelting,EBM）高孔隙桿系結構模型，設計孔隙率93.61%八面體嵌套結構模型，設計孔隙率91.48%蜂窩狀結構模型，設計孔隙率89.72%孔隙結構設計正六面體嵌套結構模型，設計孔隙率93.53%交叉曲面結構模型，設計孔隙率88.87%正交結構模型，設計孔隙率86.5%材料：不銹鋼鈦及其合金鈷鉻鉬合金粉體尺寸：45-100μm(EBM)20-30μm(SLM)金屬3D打印制造高孔隙桿系結構模型八面體嵌套結構模型蜂窩狀結構模型正交結構模型正六面體嵌套結構模型交叉曲面結構模型多孔鈦合金結構體孔隙結構特征XiangLi*,etal.Materialsletters2009;63:403-405.XiangLi*,etal.Proc.IMechEPartH:J.EngineeringinMedicine,223:173-178,2009.XiangLi*,etal.RapidPrototypingJournal,16:44-49,2010.EBM技術制造SLM技術制造仿生結構模型孔隙結構特征EBM技術制造SLM技術制造孔隙結構特征孔隙率：66-88%孔的尺寸：415-672μm桿的長度：224±43μm桿的寬度：67-187μmShimkoDA,etal.EffectofPorosityontheFluidFlowCharacteristicsandMechanicalPropertiesofTantalumScaffolds.JBiomedMaterResPartB:ApplBiomater73B:315–324,2005孔隙結構特征多孔鉭（Zimmer）力學性能測試多孔鈦P（%）65-73Zimmer63-68ActiporeSevillaP,AparicioC,PlanellJA,GilFJ.Comparisonofthemechanicalpropertiesbetweentantalumandnickel–titaniumfoamsimplantmaterialsforboneingrowthapplications.JournalofAlloysandCompounds439(2007)67–73.彈性模量E(GPa)屈服強度σo(MPa)抗壓強度σmax(MPa)孔隙率(%)1.7343.655.5851.8654.866.5813.8598.6125.8733.98155.3185.9674.72163.7197.46210-30104-121193-205Corticalbone表面改性多孔鈦在SBF模擬體液中浸泡14天SEMmorphologiesofcellsonporoustitaniumsamplesafter14daysofculture.(A–B)pureporoustitaniumscaffoldsand(C–D)poroustitaniumscaffoldswithbiomimeticcoating體外細胞培養Cellviabilityofthesampleover14daysofinvitroculture動物實驗植入兔顱骨4、8和12周后取樣Histologicalstainingforosteogenesiswithinporoustitaniumafterimplantationfor4,8and12weeks.Ti:pureporousTi,TiC:biomimeticcoatedporousTi4W×168W×1612W×1612W×100TiTiC動物實驗HistomorphometricanalysisofnewboneformationXiangLi,etal.EvaluationofBiologicalPropertiesofElectronBeamMeltedTi6Al4VImplantwithBiomimeticCoatinginVitroandInVivo.PLoSONE2012;

7(12):e52049.動物實驗利用化學氣相沉積法制備鉭涂層鉭涂層制備方法鉭涂層多孔鈦體外細胞培養實驗多孔鈦支架鉭涂層支架體外細胞培養實驗動物實驗Representativehistologicalsectionsofporousimplantsafter12weeksofimplantationinthecristailiacaofgoats.(a–c)Uncoatedand(d–f)Ta-coatedimplants.動物實驗ResultsofhistomorphometricalanalysesofuncoatedandTa-coatedXiangLi,etal.TantalumcoatingonporousTi6Al4Vscaffoldusingchemicalvapordepositionandpreliminarybiologicalevaluation.MaterialsScienceandEngineeringC2013;33:2987–2994.與組織工程相結合骨誘導生物材料﹢多孔鈦合金﹢干細胞/BMP多孔鈦合金承載體組織工程復合多孔支架多孔鈦合金支架聚合物海綿優異的力學性能組織親和性PorousTiSoakedinpolymersolutionTi/polymerconstructPorouscompositescaffoldFreezingLyophilization復合型骨修復體3DPrintingFreeze-dryingGoodMechanicalPropertiesFavorableCellAffinityalginateChitosanChitosan/HAChitosan微觀結構XiangLi,YunLuo,ChengtaoWang.PreparationandcharacterizationofporousTi6Al4V/alginatehybridimplantbycombinationofelectronbeammeltingandfreeze-drying.MaterialsLetters,2012;81:23-26.MicrostructureofTi6Al4V/silkfibroincompositescaffold體外細胞培養實驗PureTiTi+ChsTi+Chs+HAPureTiTi+ChsTi+Chs+HACellmorphologyonscaffoldsfor7and14days動物實驗羊髂骨缺損12周取樣動物實驗動物實驗小結金屬3D