標準解讀

《GB/T 26350-2026 蠅類抗藥性檢測方法 家蠅生物測定法》相較于《GB/T 26350-2010 蠅類抗藥性檢測方法 家蠅生物測定法》，在內容上進行了多方面的更新和調整，具體變化如下：

  1. 術語定義的更新：新版標準中對一些專業術語進行了更加明確的定義或新增了一些術語，以適應當前科學研究和技術發展的需求。例如，對于“抗藥性”、“敏感基線”的定義可能有所細化或擴展。

  2. 實驗材料與設備要求的變化：針對實驗所需材料（如供試昆蟲、藥劑等）以及使用的儀器設備（如培養箱、噴霧器等），2026版標準可能會提出更嚴格的質量控制要求或推薦使用新型號設備，以確保測試結果的一致性和準確性。

  3. 操作流程優化：新版本可能根據近年來的研究成果及實踐經驗，對原有實驗步驟進行了簡化或改進，比如調整了特定條件下家蠅暴露于待測藥物的時間長度、濃度梯度設置等方面，使得整個檢測過程更為高效且易于操作。

  4. 數據分析方法更新：隨著統計學方法的發展，《GB/T 26350-2026》很可能引入了新的數據處理技術或者分析軟件來提高計算效率和精確度。此外，也可能增加了更多關于如何正確解讀實驗數據的指導信息。

  5. 安全防護措施加強：考慮到實驗室安全和個人健康保護的重要性，2026版標準或將加強對實驗人員的安全培訓要求，并詳細規定了實驗過程中應采取的具體防護措施，包括但不限于穿戴適當的個人防護裝備、妥善處置廢棄物等。

  6. 環境因素考量增加：新版標準或許會更加重視外界環境條件對實驗結果的影響，在實驗設計階段就充分考慮溫度、濕度等因素，并給出相應建議或限制條件，從而保證不同實驗室之間結果具有良好的可比性。


如需獲取更多詳盡信息，請直接參考下方經官方授權發布的權威標準文檔。

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文檔簡介

ICS11.020

CCSC05

中華人民共和國國家標準

GB/T26350—2026

代替GB/T26350—2010

蠅類抗藥性檢測方法

家蠅生物測定法

Testmethodsofflyresistancetoinsecticides—

ThebioassaymethodsforMuscadomestica

2026?07?02發布2027?02?01實施

國家市場監督管理總局

國家標準化管理委員會發布

GB/T26350—2026

目次

前言··························································································································Ⅲ

1范圍·······················································································································1

2規范性引用文件········································································································1

3術語和定義··············································································································1

4試蟲·······················································································································2

4.1成蟲·················································································································2

4.2幼蟲·················································································································2

5試劑·······················································································································2

6儀器設備·················································································································2

7測試條件·················································································································2

8敏感基線法··············································································································2

8.1原理·················································································································2

8.2操作步驟···········································································································3

8.3死亡判斷···········································································································4

8.4數據處理···········································································································4

8.5結果表述···········································································································5

8.6結果判定···········································································································5

9診斷劑量法··············································································································5

9.1原理·················································································································5

9.2操作步驟···········································································································5

9.3死亡判斷···········································································································6

9.4結果表述···········································································································6

9.5結果判定···········································································································6

附錄A（資料性）常用殺蟲劑對敏感品系家蠅的致死劑量（濃度）············································7

Ⅰ

GB/T26350—2026

前言

本文件按照GB/T1.1—2020《標準化工作導則第1部分：標準化文件的結構和起草規則》的規

定起草。

本文件代替GB/T26350—2010《蠅類抗藥性檢測方法家蠅生物測定法》，與GB/T26350—

2010相比，除結構調整和編輯性改動外，主要技術變化如下：

a）增加了術語“敏感品系”“待測種群”“人工飼料混藥法”及其定義（見3.1、3.2、3.6），刪除了術語

“F1代”（見2010年版的2.2），更改了術語“診斷劑量”（見3.3，2010年版的2.1）；

b）更改了試蟲、試劑和儀器設備的要求（見第4章~第6章，2010年版的第3章~第5章）；

c）更改了測試條件（見第7章，2010年版的第6章）；

d）更改了敏感基線法中藥膜法、點滴法的操作步驟（見8.2.1、8.2.2，2010年版的7.2）；

e）更改了診斷劑量法中藥膜法、點滴法的操作步驟（見9.2.1、9.2.2，2010年版的8.2）；

f）增加了敏感基線法中人工飼料混藥法的操作步驟（見8.2.3）；

g）增加了幼蟲的死亡結果判定（見8.3.2）；

h）增加了數據處理的表述（見8.4）；

i）增加了診斷劑量法中人工飼料混藥法的操作步驟（見9.2.3）。

請注意本文件的某些內容可能涉及專利。本文件的發布機構不承擔識別專利的責任。

本文件由中華人民共和國國家衛生健康委員會提出并歸口。

本文件起草單位：北京市疾病預防控制中心、中國農業大學、揚州大學、中國疾病預防控制中心傳

染病預防控制所。

本文件主要起草人：李靜、高希武、錢坤、周小潔、孟鳳霞、佟穎、曾曉芃、尤春梅、宋敦倫。

本文件及其所代替文件的歷次版本發布情況為：

——2011年首次發布為GB/T26350—2010；

——本次為第一次修訂。

Ⅲ

GB/T26350—2026

蠅類抗藥性檢測方法

家蠅生物測定法

1范圍

本文件規定了家蠅（Muscadomestica）對有機磷類、氨基甲酸酯類、擬除蟲菊酯類、新煙堿類等殺蟲劑

抗藥性檢測時，試蟲、儀器設備和測試條件的要求，描述了敏感基線法和診斷劑量法的生物測定方法。

本文件適用于家蠅對殺蟲劑抗藥性的實驗室生物測定。

2規范性引用文件

本文件沒有規范性引用文件。

3術語和定義

下列術語和定義適用于本文件。

3.1

敏感品系susceptiblestrain

由于未曾接觸過殺蟲劑而保持對殺蟲劑具有敏感狀態，遺傳上較均一的試蟲種群。

3.2

待測種群testpopulation

從野外（現場）特定地點和時間采集的、需要進行殺蟲劑敏感性測試的目標昆蟲群體。

3.3

診斷劑量diagnosticdose

99%99.9%[99%

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